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美国SignaGen转染试剂—PolyJet™

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发表于 2011-3-2 22:38:00 | 显示全部楼层 |阅读模式
Description
Based on our proprietary polymer synthesis technology, PolyJet™ dna In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293,
COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.
Features
- Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Equally good for very long DNAs (>89 kb)

- Equally good for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple & robust transfection procedure
- Very affordable

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.


Testimonials:

PolyJet Transfection Reagent worked equally as well as lipofectamine 2000, with little evidence of cell death on 293, PC-3 and 22RV1 cells. I will defiantly consider switching over.

-----Tiffany Wallace, Ph.D., June 9, 2009, NCI / NIH

I only did side by side with the testing sample (PolyJet) and Lipo2000 with GFP transfection on COS-7 cells. The result was very good. PolyJet was even better than L2K.
------Feng Qiao, Ph.D., Jun 12, 2009, NEI / NIH

I tested the sample of PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results (I did not quantify the % transfection efficiency, but the pictures get the point across). I found that the protocol for difficult-to-transfect cell lines worked better than the standard protocol.
-----Stephanie Murphy, Ph.D., June 29, 2009, Dartmouth College

I had chance to try your product finally. It was great success. I used HeLa cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you! I was wondering if I also try GenJet™ Plus DNA In Vitro Transfection Reagent? According to your website, the reagent works better than regular PolyJet.
-------Yumi Uetake, Ph.D., August11, 2009, UMASS

I tested PolyJet and it looks great on MDCK. We placed order. Thank you!
-------Ge Zhou, Ph.D., August 12, 2009, NYU

We are happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we got approximately 80% efficiency with pMAX GFP plasmid, by following the conditions in your suggested protocol. We ran a comparison with Lipofectamine, which only showed approximately 20-30% transfection efficiency. We are planning experiments and will be ordering more soon.
-------Emily Mcallister, September 3rd, 2009, PBRC
 


中国总经销:济南美中清水湾生物科技有限公司
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