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[求助] 关于TGI Clustering tools ---TGICL的一个问题

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发表于 2011-3-14 20:48:13 | 显示全部楼层 |阅读模式
TGI Clustering tools (TGICL): a software system for fast clustering of large EST datasets

This package automates clustering and assembly of a large EST/mRNA dataset. The clustering is performed by a slightly modified version of NCBI's megablast , and the resulting clusters are then assembled using CAP3 assembly program. TGICL starts with a large multi-FASTA file (and an optional peer quality values file) and outputs the assembly files as produced by CAP3. Both clustering and assembly phases can be parallelized by distributing the searches and the assembly jobs across multiple CPUs, as TGICL can take advantage of either SMP machines or PVM (Parallel Virtual Machine) clusters.

TGICL进行序列拼接真的很不错,可是我在使用该软件的时候产生的singletons里却包含了我所有的拼接前的序列名称,按照说明书上,应该是只有singletons啊,也就是说排除了拼接形成contig的那部分序列。不知道大家有没有用过这个软件,看我出错在什么地方了。
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